Arterial stiffness is associated with adipokine dysregulation in non-hypertensive obese mice

作者：Gil Ortega Marta; Martin Ramos Miriam; Arribas Silvia M; Carmen Gonzalez M; Aranguez Isabel; Ruiz Gayo Mariano; Somoza Beatriz^*; Fernandez Alfonso Maria S

来源：Vascular Pharmacology, 2016, 77: 38-47.

DOI：10.1016/j.vph.2015.05.012

• Diet-induced obesity
• Arterial stiffness
• Adipokine
• Arterial remodeling
• Oxidative stress

The aim of this study was to characterize alterations in vascular structure and mechanics in murine mesenteric arteries from obese non-hypertensive mice, as well as their relationship with adipokines. Four-week old C57BL/6J male mice were assigned either to a control (C, 10% kcal from fat) or a high-fat diet (HFD, 45% kcal from fat) for 32 weeks. HFD animals weighed 30% more than controls (p < 0.001), exhibited similar blood pressure, increased leptin, insulin and superoxide anion (O-2(center dot-)) levels, and reduced adiponectin levels and nitric oxide (NO) bioavailability. Arterial structure showed an outward remodeling with an increase in total number of both adventitial and smooth muscle cells in HFD. Moreover, HFD mice exhibited an increased arterial stiffness assessed by beta-values (C = 2.4 +/- 0.5 vs HFD = 5.3 +/- 0.8; p < 0.05) and aortic pulse wave velocity (PWV, C = 3.4 +/- 0.1 vs HFD = 3.9 +/- 0.1; p < 0.05). beta-Values and PWV positively correlated with leptin, insulin or O-2(center dot-) levels, whereas they negatively correlated with adiponectin levels and NO bioavailability (p < 0.01). A reduction in fenestrae number together with an increase in type-I collagen amount (p < 0.05) were observed in HFD. These data demonstrate that HFD accounts for the development of vascular remodeling and arterial stiffness associated with adipokine dysregulation and oxidative stress, independently of hypertension development.

• 出版日期2016-2