A multi-purpose flow manifold for the spectrophotometric determination of sulphide, sulphite and ethanol involving gas diffusion: Application to wine and molasses analysis

作者：Silva Claudineia R; Gomes Taciana F; Barros Valdemir A F; Zagatto Elias A G^*

来源：Talanta, 2013, 113: 118-122.

DOI：10.1016/j.talanta.2013.03.021

• Flow analysis
• Multi-purpose flow manifold
• Sulphide
• Sulphite and ethanol
• Wine and molasses analysis
• Spectrophotometry

A simple and rugged flow set up was designed for spectrophotometric determination of sulphide, sulphite and ethanol aiming at quality assessment of wines, control of industrial fermentation, and selection of yeast strain. The different assays involved gas diffusion through a Teflon planar membrane and were carried out after minor modifications in the manifold, namely reagent composition and total flow rate. Main figures of merit: linear analytical curves=0.50-6.0 mgL(-1)S(2-), 2.5-20.0 mg L-1 SO3- and 5.0-25.0% (v/v) of ethanol; detection limits (3 sigma)=0.035 mg L-1 S2-, 0.2 mg L-1 SO3- and 0.18% (v/v) of ethanol; peak height r.s.d.=2.18% for 4.03 mg L-1 S2- spiked molasses, 2.21% for a 9.82 mg L-1 SO3- wine and 2.07% for a typical wine (12.53% v/v of ethanol), sampling rate=15, 57 and 29 h(-1), reagent consumptions=1.9 mu mol of N,N-dimethyl-p-phenylenediamine, 1.68 mu g of Malachite green and 0.68 mmol Cr(VI) per determination, respectively.

• 出版日期2013-9-15