Age-related changes of CD4(+) T cell migration and cytokine expression in germ-free and SPF mice periodontium

作者：Irie Koichiro^*; Tomofuji Takaaki; Ekuni Daisuke; Fukuhara Daiki; Uchida Yoko; Kataoka Kota; Kobayashi Shuichiro; Kikuchi Takeshi; Mitani Akio; Shimazaki Yoshihiro; Morita Manabu

来源：Archives of Oral Biology, 2018, 87: 72-78.

DOI：10.1016/j.archoralbio.2017.12.007

• Host response
• Inflammation and innate immunity
• Pathogenesis of periodontal disease
• T-cell biology

Objective: Increasing age is a potential risk factor for periodontal tissue breakdown, which may be affected by commensal flora. The aim of this study evaluated age-related changes in CD4 (+) T cells, C-C chemokine ligand 5 (CCL5), interleukin (IL)-17A, and receptor activator of nuclear factor-kappa B ligand (RANKL) expression using germ-free (GF) and conventionally reared (SPF) mice.
Design: GF and SPF mice at 8 (n = 6/group) and 22 weeks old (n = 6/group) were used. Immunohistochemical analyses were performed to determine the effects of aging on protein expression in periodontal tissues. Age related changes in alveolar bone were quantified using micro-CT analysis.
Results: SPF mice, but not GF mice, showed an age-related increase in alveolar bone loss (P < 0.01). SPF mice at 22 weeks of age increased expression of GD4 (+) T cells, CCL5, IL-17A, and RANKL compared to those at 8 weeks of age in connective tissue and alveolar bone surface (P < 0.01). Furthermore, there was increased CD4 (+) T cells, which were co-expressed with IL-17A and RANKL in SPF mice at 22 weeks of age. On the other hand, the GF mice did not show any significant differences in CD4 (+) T cells, CCL5, IL-17A and RANKL expression between the two age groups.
Conclusions: SPF mice induced an age-related increase in CD4 (+) T cells co-expressed with IL-17A and RANKL, with occurring alveolar bone loss. In contrast, GF mice did not show age-related changes in CD4 (+) T cell migration and cytokine expression.

• 出版日期2018-3