Determination of vitamin B-6 (pyridoxine hydrochloride) based on a novel BZ oscillating reaction system catalyzed by a macrocyclic complex

作者：Zeng Qingling^*; Chen Lulu; Song Xianyi; Hu Gang; Hu Lin

来源：CENTRAL EUROPEAN JOURNAL OF CHEMISTRY, 2014, 12(3): 325-331.

DOI：10.2478/s11532-013-0383-4

• Determination
• Oscillating reaction
• Macrocyclic complex
• Vitamin B-6

This paper reports a new method for determination of VB6 (pyridoxine hydrochloride) by its perturbation effects on a novel Belousov-Zhabotinskii (BZ) oscillating system. This novel BZ system, in which malic acid serves as the substrate, contains an enzyme-like complex, macrocyclic complex {[CuL](ClO4)(2)}, as catalyst. The ligand L in the complex is 5,7,7,12,14,14-hexamethyl-1,4,8,11-tetraazacyclotetradeca-4,11-diene. Results show that the addition of pyridoxine hydrochloride can perturb the oscillation amplitude and period, and the change of the oscillation amplitude is linearly proportional to the concentration of pyridoxine hydrochloride in the range of 5x10(-7)-2.5x10(-4) M. The obtained RSD with seven samples is 3.073%. An assay of pharmaceutical tablets of vitamin B-6 was evaluated. Some foreign ions were studied with respect to their possible influence on the determination of pyridoxine hydrochloride. The factors which influence this reaction include the concentration of reactant, the temperature of the reaction, property of catalyst, etc. Furthermore, the possible reaction mechanism has been proposed using the Field-Koros-Noyes (FKN) model.

• 出版日期2014-3
• 单位安徽大学; 中国科学院长春应用化学研究所