Analysis of mercury and selenium during subcritical water treatment of fish tissue by various atomic spectrometric methods

作者：Ohki Akira^*; Hayashi Kentaro; Ohsako Joji; Nakajima Tsunenori; Takanashi Hirokazu

来源：Microchemical Journal, 2013, 106: 357-362.

DOI：10.1016/j.microc.2012.09.008

• Subcritical water
• Fish tissue
• Dissolution
• Methylmercury
• Selenium

Subcritical water treatment of biomass and food waste (especially marine product) is now very attractive in terms of the production of useful chemicals. Big marine fish, such as tuna, usually contain a high level of Hg, which is considered safe because of the sequestration by Se species. In this study, such fish tissues were treated with subcritical water, and the resulting aqueous phase was analyzed for Hg and Se to estimate the dissolution behavior of these toxic elements. The determination of total Hg in the aqueous phase was carried out by heat vaporization atomic absorption spectrometry (HVAAS), while that of Se was done by inductively coupled plasma mass spectrometry (ICP-MS). The dissolution behavior of those elements was favorably monitored. The speciation of Hg in the aqueous phase was performed by HPLC with cold vapor atomic fluorescence spectrometry aided by UV irradiation-oxidation (HPLC-UV-CVAFS), and the Hg species detected was mainly methylated Hg. For the speciation of Se, size-exclusion chromatography (SEC) with ICP-MS was attempted to use, and it was found that high molecular and insoluble Se species, which were originally contained in the tissue, was gradually decomposed into lower molecular hydrophilic species, and dissolved in the aqueous phase.

• 出版日期2013-1