An analysis of polymer type and chain length for use as a biological composite graft extender in impaction bone grafting: A mechanical and biocompatibility study

作者：Tayton Edward; Fahmy Sherif; Purcell Matthew; Aarvold Alexander; Smith James O; Kalra Spandan; Briscoe Adam; Lanham Stuart; Howdle Steven; Shakesheff Kevin; Dunlop Douglas G; Oreffo Richard O C^*

来源：Journal of Biomedical Materials Research Part A, 2012, 100A(12): 3211-3219.

DOI：10.1002/jbm.a.34264

• stem cells
• polymer
• scaffolds
• impaction bone grafting
• tissue engineering

Impaction bone grafting (IBG) with human allograft remains the preferred approach for replacement of lost bone stock during revision hip surgery. Associated problems include cost, disease transmission, and stem subsidence. Synthetic grafts are therefore appealing, and ideally display similar mechanical characteristics as allograft, but with enhanced ability to form de novo bone. High and low molecular weight forms of three different polymers [poly(DL-lactide) (PDLLA), poly(DL-lactide-co-glycolide) (PDLLGA), and poly(e-caprolactone) (PCL)] were milled, impacted into discs, and then examined in a shear testing rig, in comparison to allograft. In addition, skeletal stem cells (SSCs) were combined with each of the milled polymers, followed by impaction and examination for cell viability and number, via fluorostaining and biochemical assays. The shear strengths of high/low mwt PDLLA, and high/low mwt PDLLGA were significantly higher than allograft (p %26lt; 0.01). High/low mwt PCL had significantly lower shear strengths (p %26lt; 0.01). WST-1 assay and fluorstaining indicated significantly increased cell viability on high mwt PDLLA and high mwt PDLLGA over allograft (p %26lt; 0.05). Mechanical and biochemical analysis indicated improved properties of high mwt PDLLA and high mwt PDLLGA over allograft. This study indicates the potential of these polymers for use as substitute human allograft, creating a living composition with SSC for application in IBG.

• 出版日期2012-12