科研之友
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摘要
The emergence of pan-resistance in bacterial pathogens poses a threat to human health. Carbapenem-resistant Acinetobacter baumannii has emerged as a serious challenge, causing nosocomial infection and community-acquired outbreaks in hospitals globally, including in Pakistan. We collected 90 Acinetobacter isolates from patients with secondary or nosocomial infections from different hospitals in Pakistan and screened for carbapenem-resistant strains. Of the 90 isolates, 59 were resistant to carbapenems. Among oxacillinase-encoding genes, bla(OXA-51)-like was common in all isolates, including in combination with bla(OXA-23)-like in 14 isolates; however, bla(OXA-24)-like and bla(OXA-58)-like were completely absent. Among metallo-beta-lactamase-encoding genes, only bla(NDM-1) was found in one isolate, while the other three genes, bla(IMP), bla(VIM) and bla(SIM), were completely absent. None of the isolates was found to harbour the bla(CTX-M) gene. The isolates were also tested for susceptibilities to a panel of different antibiotics belonging to several classes. Of all the drugs tested, tigecycline was the most effective with 80% sensitivity amongst isolates, followed by colistin with 50% sensitivity. Three categories of resistance were found in these isolates: extreme drug resistance in 26, pan-drug resistance in 19 and multidrug resistance in 87 isolates. The isolates exhibited a high resistance to cephalosporins, trimethoprim sulfamethoxazole and beta-lactam antibiotics, followed by tetracycline and beta-lactam/beta-lactam inhibitor combination, fluoroquinolone and aminoglycosides. The results show a prominent level of antibiotic-resistance phenotypes in A. baumannii and strongly suggest the need for full-scale national surveillance of carbapenem-resistant A. baumannii with particular emphasis on the newly identified NDM-1 (New Delhi metallo-beta-lactamase-1).
- 出版日期2014-1
