Development of a Pichia pastoris whole-cell biocatalyst with overexpression of mutant lipase I PCLG47I from Penicillium cyclopium for biodiesel production

作者：Liu, Yihan^*; Huang, Lin; Zheng, Dong; Fu, Yu; Shan, Mengying; Xu, Zehua; Ma, Jieying; Lu, Fuping^*

来源：RSC Advances, 2018, 8(46): 26173-26180.

DOI：10.1039/c8ra04462g

• LITOPENAEUS-VANNAMEI HEPATOPANCREAS
• IMMOBILIZED LIPASE
• SURFACE DISPLAY
• OPTIMIZED SYNTHESIS
• FUEL PRODUCTION
• OIL
• TRANSESTERIFICATION
• PERSPECTIVES
• IMPROVEMENT
• MICROALGAE

Penicillium cyclopium lipase I (PCL) is a thermolabile triacylglycerol lipase with very low activity against monoacylglycerols, and there have been no reports on the transesterification of oil to produce biodiesel. A mutant PCLG47I with an improved thermostability was previously obtained through replacing Gly47 with Ile in PCL. In this study, a novel Pichia pastoris whole-cell biocatalyst (WCB) with overexpression of PCLG47I was constructed and characterized for biodiesel production from soybean oil. The optimum conditions for biodiesel preparation were 1 g soybean oil, 1 : 2 initial oil/methanol molar ratio with 3 times methanol addition of 1 : 0.75 oil/methanol molar ratio at 4 h intervals, 7% water content, 400 U lipase, temperature of 25 degrees C, and reaction time of 20 h. Under the optimum conditions, the FAME yield reached 60.7% and remained 47.3% after 4 batch cycles, and no glycerol was generated as a byproduct. These findings indicated that this WCB is a promising biocatalyst for biodiesel production in a relatively cost-effective manner. Additionally, the resulting enzymatic process may provide a potential method for biodiesel production at an industrial scale.

• 出版日期2018
• 单位天津科技大学