Alterations in B-cell subsets in pediatric patients with early atopic dermatitis

作者：Czarnowicki Tali^*; Esaki Hitokazu; Gonzalez Juana; Renert Yuval Yael; Brunner Patrick; Oliva Margeaux; Estrada Yeriel; Xu Hui; Zheng Xiuzhong; Talasila Sreya; Haugh Isabel; Thy Huynh; Lyon Sarah; Tran Gary; Sampson Hugh; Suarez Farinas Mayte; Krueger James G; Guttman Yassky Emma; Paller Amy S

来源：Journal of Allergy and Clinical Immunology, 2017, 140(1): 134-+.

DOI：10.1016/j.jaci.2016.09.060

• Atopic dermatitis
• B cells
• T cells
• allergen-specific IgE
• atopic march

Background: B cells undergo maturation and class-switching in response to antigen exposure and T-cell help. Early B-cell differentiation has not been defined in patients with early-onset atopic dermatitis (AD).
Objective: We sought to define the frequency of B-cell subsets associated with progressive B-cell maturation and IgE class-switching.
Methods: We studied 27 children and 34 adults with moderate-to-severe AD (mean SCORAD score, 55 and 65, respectively) and age-matched control subjects (15 children and 27 adults). IgD/CD27 and CD24/CD38 core gating systems and an 11-color flow cytometric panel were used to determine the frequencies of circulating B-cell subsets. Serum total and allergen-specific IgE (sIgE) levels were measured by using ImmunoCAP.
Results: Compared with adults, children showed T-cell predominance in the skin. Circulating CD19(+) CD20(+) B-cell counts were lower in patients with pediatric AD than in control subjects (24% vs 33%, P = .04), whereas CD3(+) T-cell counts were higher (62% vs 52%, P = .05). A decreased B-cell/T-cell lymphocyte ratio with age was observed only in pediatric control subjects (r = -0.48, P = .07). In pediatric patients with AD, a positive correlation was observed between B-cell/T-cell ratio and nonswitched memory B-cell counts (r = 0.42, P = .03). Higher frequencies of positive sIgE levels were seen in pediatric patients with AD (P < .0001). Diverse sIgE levels correlated with SCORAD scores and age of pediatric patients with AD (P < .01). Positive correlations were observed between activated B-cell and memory T-cell counts (P < .02). In patients with AD, IgE sensitization to most allergens clustered with age, T(H)1, T(H)2, total IgE levels, and B-cell memory subsets.
Conclusions: Peripheral B and T cells are altered in pediatric patients with early AD, but T cells predominate in skin lesions.

• 出版日期2017-7
• 单位西北大学