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摘要
Response surface methodology was used to evaluate the quantitative effects of two independent variables, namely solvent polarity and ratio of ultrasound power to sonication time, on the antioxidant capacity and total phenolics content (TPC) in two spring rapeseed cultivars: Feliks and Markus. The mean FRAP, DPPH, and TPC results ranged between 1082-5104 mu mol Trolox (TE)/100g and 1203-5528 mu mol TE/100g, 5373-17417 mu mol TE/100g and 3015-15 989 mu mol TE/100g, 252-1258mg sinapic acid (SA)/100g and 282-1292mg SA/100g for Feliks and Markus varieties, respectively. The quadratic effect of solvent polarity on FRAP, DPPH, and TPC in the examined extracts was about 11, 2, and 7 times greater, respectively, than the quadratic effect of ultrasound power to sonication time ratio. The predicted optimum solvent polarity=56.8 and 55.6, and US power/US time=4.0 and 4.2W/min resulted in FRAP=5143 mu mol TE/100g and 5503 mu mol TE/100g, DPPH=16 623 mu mol TE/100g and 15 839 mu mol TE/100g, TPC=1250mg SA/100g and 1259mg SA/100g in extracts obtained from Feliks and Markus cultivars, respectively. Practical applications: Bioactive compounds extracted from rapeseed varieties provide health benefits and have antioxidative properties. The application of ultrasound resulted in an increase in antioxidant extraction from rapeseed. Therefore, it seems worth while to consider the application of ultrasound in the fat industry for the production of rapeseed oils with a high content of antioxidants. The ultrasound-assisted extraction of antioxidants from rapeseed would make it possible to improve technological processes and consequently reduce processing times and costs. Moreover, the analytical methods used, FRAP, DPPH, and Folin-Ciocalteu, do not require specialized equipment and can be employed by the fat industry laboratories to assess the antioxidant capacity and total phenolics in rapeseed cultivars.
- 出版日期2015-4
