Abnormal Platelet Amyloid-beta Protein Precursor (A beta PP) Metabolism in Alzheimer's Disease: Identification and Characterization of a New A beta PP Isoform as Potential Biomarker

作者：Jelic Vesna; Hagman Goran; Yamamoto Natsuko Goto; Teranishi Yasuhiro; Nishimura Takeshi; Winblad Bengt; Pavlov Pavel F^*

来源：Journal of Alzheimer's Disease, 2013, 35(2): 285-295.

DOI：10.3233/JAD-122122

• Alzheimer's disease
• amyloid-beta protein precursor
• biomarker
• platelets

Previous findings demonstrated an altered pattern of amyloid-beta protein precursor (A beta PP) expression in platelets of Alzheimer's disease (AD) patients compared with either healthy control subjects or patients with non-Alzheimer-type dementia. In an attempt to explore the diagnostic potential of platelet A beta PP metabolism, we have generated monoclonal antibodies directed to the N-terminal part of A beta PP. We have observed two different antibody recognition patterns of A beta PP: one resembling previously described 130 kDa and 105 kDa species and a novel A beta PP 115 kDa form. This form was significantly increased in platelets of the mild cognitive impairment and AD group as compared to control subjects. The abundance of A beta PP 115 kDa species correlated with the previously described A beta PP 130/105 kDa ratio as well as with Mini-Mental State Examination score. Despite the inability of these particular monoclonal antibodies to recognize native forms of A beta PP, identification of a new A beta PP isoform in platelets as a potential AD biomarker can provide an additional tool for the development of a reliable diagnostic test to detect preclinical stages of AD.

• 出版日期2013