A Dual-Modality System for Both Multi-Color Ultrasound-Switchable Fluorescence and Ultrasound Imaging

作者：Kandukuri Jayanth; Yu Shuai; Cheng Bingbing; Bandi Venugopal; D%apos; Souza Francis; Nguyen Kytai T; Hong Yi; Yuan Baohong

来源：International Journal of Molecular Sciences, 2017, 18(2): 323.

DOI：10.3390/ijms18020323

• ultrasound-switchable fluorescence imaging
• ultrasound imaging
• dual-modality imaging
• multi-color fluorescence imaging
• deep-tissue fluorescence imaging

Simultaneous imaging of multiple targets (SIMT) in opaque biological tissues is an important goal for molecular imaging in the future. Multi-color fluorescence imaging in deep tissues is a promising technology to reach this goal. In this work, we developed a dual-modality imaging system by combining our recently developed ultrasound-switchable fluorescence (USF) imaging technology with the conventional ultrasound (US) B-mode imaging. This dual-modality system can simultaneously image tissue acoustic structure information and multi-color fluorophores in centimeter-deep tissue with comparable spatial resolutions. To conduct USF imaging on the same plane (i.e., x-z plane) as US imaging, we adopted two 90 degrees-crossed ultrasound transducers with an overlapped focal region, while the US transducer (the third one) was positioned at the center of these two USF transducers. Thus, the axial resolution of USF is close to the lateral resolution, which allows a point-by-point USF scanning on the same plane as the US imaging. Both multi-color USF and ultrasound imaging of a tissue phantom were demonstrated.

• 出版日期2017-2