Augmented biogas production from protein-rich substrates and associated metagenomic changes

作者：Kovacs Etelka; Wirth Roland; Maroti Gergely; Bagi Zoltan; Nagy Katalin; Minarovits Janos; Rakhely Gabor; Kovacs Kornel L^*

来源：Bioresource Technology, 2015, 178: 254-261.

DOI：10.1016/j.biortech.2014.08.111

• Anaerobic degradation
• Biogas
• Protein-rich waste
• Metagenomics
• Bioaugmentation

This study demonstrates that appropriate adaptation of the microbial community to protein-rich biomass can lead to sustainable biogas production. The process of acclimation to these unusual mono-substrates was controlled by the protease activity of the microbial community. Meat extract (C/N = 3.32) and kitchen waste (C/N = 12.43) were used as biogas substrates. Metagenome analysis highlighted several mesophilic strains that displayed a preference for protein degradation. Bacillus coagulans, Bacillus subtilis and Pseudomonas fluorescens were chosen for detailed investigation. Pure cultures were added to biogas reactors fed solely with protein-rich substrates. The bioaugmentation resulted in a 50% increase in CH4 production even without any acclimation. The survival and biological activity of the added bacteria were followed in fed-batch fermenters by qPCR. Stable biogas production was observed for an extended period of time in laboratory CSTR reactors fed with biomass of low C/N.

• 出版日期2015-2