No effect of sustained systemic growth retardation on the distribution of Reelin-expressing interneurons in the neuron-producing hippocampal dentate gyrus in rats

作者：Ohishi Takumi; Wang Liyun; Ogawa Bunichiro; Fujisawa Kenichi; Taniai Eriko; Hayashi Hitomi; Mitsumori Kunitoshi; Shibutani Makoto^*

来源：Reproductive Toxicology, 2010, 30(4): 591-599.

DOI：10.1016/j.reprotox.2010.08.009

• Developmental growth retardation
• Maternal protein restriction
• Hippocampal dentate gyrus
• Reelin
• gamma Aminobutyric acid (GABA)ergic interneuron
• Neuronal migration
• Neurogenesis

Reelin signaling plays a role in neuronal migration and positioning during brain development To clarify the effect of systemic growth retardation on the distribution of Reelin-expressing interneurons in the hilus of the hippocampal dentate gyrus pregnant rats were fed a synthetic diet with either a normal (20% casein) or low (10% casein) protein concentration from gestational day 10 to postnatal day (PND) 21 at weaning Male offspring were immunohistochemically examined at PND 21 and on PND 77 Protein-restricted offspring displayed systemic growth retardation through PND 77 and had decreased absolute brain weights and an increased number of external granular cells in the cerebellar cortex suggestive of retarded brain growth at weaning However maternal protein restriction did not change the cellular distribution of immunoreactivity for Reelin Calbindin-D-28K or glutamic acid decarboxylase 67 or of NeuN-positive postmitotic neurons in the dentate hilus either at PND 21 or PND 77 which suggests that the population of gamma-aminobutyric acid-ergic interneurons involving synthesis of Reelin was not affected Furthermore as well as the distribution of hilar neurons expressing neurogenesis-related FoxG1 cell proliferation and apoptosis in the subgranular zone were unaffected through PND 77 These results suggest that systemic growth retardation caused by maternal protein restrict

• 出版日期2010-12