Adjuvant Effects of Dual Co-stimulatory Molecules on Cellular Responses to HIV Multiple-epitope DNA Vaccination

作者：Shen Zhen wei; Jin Hong tao; Li Chang; Cong Yan zhao; Nan Wen long; Bai Liang; Jin Ning yi^*

来源：Chemical Research in Chinese Universities, 2009, 25(3): 347-352.

• HIV DNA vaccine
• Co-stimulatory molecules
• 4-IBBL
• OX40L
• Dendritic cells

Designing adjuvants that can induce strong cytotoxic T cell responses is largely required for preparing DNA vaccines. In this study we explored dual costimulatory molecules 4-1BBL and OX40L as adjuvants to improve the efficiency of the HIV multiple-epitope DNA vaccine. When explored in the human dendritic cell-T cell based co-culture system, dual costimulatory molecules significantly enhanced the anti-HIV T cell response of the HIV multiple-epitope DNA vaccine, as detected by intracellular cytokine staining to HIV antigens, cytokines accumulation in the cultures, and antigen-specific cytotoxic T lymphocyte responses. These results suggest that dual costimulatory molecules 4-1BBL and OX40L can effectively increase the potential of the HIV multiple-epitope antigen DNA vaccine and may provide an exciting approach for HIV therapy.

• 出版日期2009-5-25
• 单位中国人民解放军军事医学科学院; 吉林大学