A small molecule chemical chaperone optimizes its unfolded state contraction and denaturant like properties

作者：Sharma Sunny; Sarkar Suparna; Paul Simanta Sarani; Roy Syamal; Chattopadhyay Krishnananda^*

来源：Scientific Reports, 2013, 3(1): 3525.

DOI：10.1038/srep03525

Protein aggregation is believed to occur through the formation of misfolded conformations. It is expected that, in order to minimize aggregation, an effective small molecule chaperone would destabilize these intermediates. To study the mechanism of a chemical chaperone, we have designed a series of mutant proteins in which a tryptophan residue experiences different local environments and solvent exposures. We show that these mutants correspond to a series of conformationally altered proteins with varying degree of misfolding stress and aggregation propensities. Using arginine as a model small molecule, we show that a combination of unfolded state contraction and denaturant like properties results in selective targeting and destabilization of the partially folded proteins. In comparison, the effect of arginine towards the folded like control mutant, which is not aggregation prone, is significantly less. Other small molecules, lacking either of the above two properties, do not offer any specificity towards the misfolded proteins.

• 出版日期2013-12-17