A Glycosidic Spinasterol from Koreana stewartia Promotes Procollagen Production and Inhibits Matrix Metalloproteinase-1 Expression in UVB-Irradiated Human Dermal Fibroblasts

作者：Lee Tae Hoon; Lee Sang Min; Lee Dae Young; Son Youngsook; Chung Dae Kyun; Baek Nam In; Kim Jiyoung^*

来源：Biological & Pharmaceutical Bulletin, 2011, 34(5): 768-773.

DOI：10.1248/bpb.34.768

• photoaging
• spinasterol
• collagen
• matrix metalloproteinase-1
• Stewartia koreana

Methanol extract of Korean stewartia leaves (SKE) stimulated collagen production in ultraviolet-B (UVB)-irradiated human fibroblast cells. An active compound was isolated from SKE by successive partitioning and chromatography, and the chemical structure was determined to be 3-O-beta-D-glucopyranosylspinasterol (spinasterol-Glc) by spectroscopic characterization. Spinasterol-Glc increased collagen production in the supernatant of UVB-irradiated dermal fibroblast cell cultures in a dose-dependent manner. The effects of spinasteol-Glc on expression of procollagen and matrix metalloproteinase-1 (MMP-1) were further evaluated. We found that the compound stimulated collagen production in UVB-treated fibroblasts than in vehicle-treated control cells by about 3-fold. In addition, we also demonstrate that the compound increased the mRNA and protein levels of procollagen in UVB-treated fibroblast cells, while it inhibited expression of MMP-1. These results indicate that spinasterol-Glc protects fibroblast cells from the adverse effects of UV radiation via stimulation of procollagen synthesis as well as inhibition of MMP-1 expression. Spinasterol-Glc may be useful in the future development of therapeutic and cosmetic applications.

• 出版日期2011-5