Antagonistic effect of Candida albicans and IFN gamma on E-cadherin expression and production by human primary gingival epithelial cells

作者：Rouabhia Mahmoud^*; Semlali Abdelhabib; Audoy Julie; Chmielewski Witold

来源：Cellular Immunology, 2012, 280(1): 61-67.

DOI：10.1016/j.cellimm.2012.11.008

• E-cadherin
• Candida albicans
• Epithelial cells
• Oral mucosa

Caused mainly by Candida albicans, oropharyngeal candidiasis is the most common oral complication associated with HIV disease worldwide. Host defenses against C. albicans essentially fall into two categories: specific immune mechanisms and local oral mucosal epithelial cell defenses. Since oral mucosa is the first line of defense in the form of a physical barrier against C. albicans invasion, and since epithelial cells are involved in anti-Candida innate immunity through different cytokines, we wanted to determine whether C. albicans alters E-cadherin expression and production, and whether interferon-gamma (INF gamma), a TH1 cytokine, is involved in the anti-Candida defense. Using primary human gingival epithelial cells, we demonstrated that C. albicans significantly decreased E-cadherin mRNA expression and protein production. This effect was basically obtained at later infective periods (24 and 48 h). Interestingly, when IFN gamma was added to C. albicans infected epithelial cell cultures, it prevented the side effect of C. albicans on E-cadherin mRNA expression and protein production and deposition. All together, these results suggested concomitant interactions between oral epithelial cells and IFN gamma against C. albicans infection.

• 出版日期2012-11