Association of polygalacturonase-inhibiting protein gene 2 (MsPGIP2) to common leaf spot resistance in alfalfa

作者：Gui, Zhi; Gao, Jianming; Xin, Nan; Wang, Yu; Pi, Yongshuo; Liu, Huiqin; Yuan, Qinghua; Li, Xianglin^*

来源：European Journal of Plant Pathology, 2016, 144(2): 245-256.

DOI：10.1007/s10658-015-0720-x

• Lucerne
• PGIPs
• Association
• Common leaf spot
• SNPs
• FSSCP

Common leaf spot (CLS), which is caused by Pseudopeziza medicaginis (Lib.) Sacc., is one of the most serious fungal diseases occurring in autotetraploid alfalfa (Medicago sativa) worldwide. Polygalacturonase-inhibiting proteins (PGIPs) are important candidate genes for disease resistance studies in plants. The association between an alfalfa PGIP gene, MsPGIP2, and the CLS resistance of alfalfa was tested using a population including 400 plants from 16 cultivars. Direct sequencing detected 21 single-nucleotide polymorphisms (SNPs) with a genotypic frequency > 0.1 over the sequenced fragment of about 635 bp. Linkage disequilibrium (LD) within this region rapidly decreased as expected and no evident structure among the studied population was observed. Two synonymous coding-region SNPs, L458 and L563, revealed weak but significant associations to the CLS resistance, mainly impacting on the spot size. Furthermore, L458 exhibited a greater effect on CLS severity than L563. Fluorescent single-strand conformational polymorphism (FSSCP) marker S331a was found to be associated to CLS resistance, and exclusively contained a favourable base to the CLS resistance in L458. Thus, MsPGIP2 could play a small role in CLS resistance of alfalfa by limiting the extension of disease spots. FSSCP marker S331a could be useful for marker-assisted selection of L458.

• 出版日期2016-2
• 单位天津农学院; 中国农业科学院