Pioglitazone, a PPAR gamma ligand, suppresses NFKB activation through inhibition of 1 kappa B kinase activation in cerulein-treated AR42J cells

作者：Wan, Hongyu; Yuan, Yaozong^*; Qian, Aihua; Sun, Yan; Qiao, Minmin

来源：Biomedicine & Pharmacotherapy, 2008, 62(7): 466-472.

DOI：10.1016/j.biopha.2007.10.012

• cerulein
• inflammation
• AR42J
• NF kappa B
• PPAR gamma
• pioglitazone
• GW9662

Background and aim: NF kappa B plays a major role in the immune and inflammation responses of pancreatitis. Recently, there is increasing evidence that the expression and activity of PPAR gamma may participate in the activity of NF kappa B. Therefore, we investigated a putative relationship of the two transcription factors in cerulein-treated pancreatic acinar AR42J cells.
Method: AR42J were stimulated by cerulein with or without the presence of a PPAR gamma activator pioglitazone or a PPAR gamma antagonist GW9662.
Results: Treatment of AR42J cells with pioglitazone attenuated cerulein induced p50 and p65 NF kappa B dimer activity in the nucleus as measured by transcription factor assay. Cytosolic expression of I kappa B alpha protein was reduced by cerulein, basal signalling was not influenced by the PPAR gamma inhibitor GW9662 and pioglitazone. Adversely, the inhibitory effect of pioglitazone on NF kappa B activity induced by cerulein was almost reversed by GW9662.
Conclusion: These findings provide evidence for the involvement of the nuclear hormone receptors PPAR gamma in the activity Of NF kappa B in cerulein-treated AR42J cells.

• 出版日期2008-9
• 单位上海市闵行区中心医院; 上海交通大学