Ni(II)-NTA Modified Poly(ethylene imine) Glycopolymers: Physicochemical Properties and First In Vitro Study of Polyplexes Formed with HIV-Derived Peptides

作者：Hauptmann Nicole; Pion Marjorie; Munoz Fernandez Maria Angeles; Komber Hartmut; Werner Carsten; Voit Brigitte^*; Appelhans Dietmar

来源：Macromolecular Bioscience, 2013, 13(5): 531-538.

DOI：10.1002/mabi.201200449

• dendritic cells
• dendritic glycopolymers
• NTA ligands
• poly(ethylene imine)
• polyplexes

Alternative delivery entities are desirable in immunotherapies in which polyplexes are widely formed by electrostatic interactions to induce cellular uptake processes for bioactive molecules. In our study, biocompatible Ni(II)-nitrilo(triacetic acid)-modified poly(ethylene imine)-maltose (Ni-NTA-DG) is realized and evaluated as complexation agent against His-tagged peptides using fluorescence polarization and dynamic light scattering. The polyplexes are stable until a pH of 6.56.0, and also up to 50mM of imidazole. A first uptake approach shows that polyplexes lead to an increase in peptide uptake in monocyte-derived immature dendritic cells. In summary, Ni-NTA-DG represents a promising (delivery) platform for forthcoming in vitro applications.

• 出版日期2013-5