Soybean glyceollins mitigate inducible nitric oxide synthase and cyclooxygenase-2 expression levels via suppression of the NF-kappa B signaling pathway in RAW 264.7 cells

作者：Yoon Eun Kyung; Kim Hyun Kyoung; Cui Song; Kim Yong Hoon; Lee Sang Han^*

来源：International Journal of Molecular Medicine, 2012, 29(4): 711-717.

DOI：10.3892/ijmm.2012.887

• glyceollins
• NF-kappa B
• inducible nitric oxide synthase
• cyclooxygenase-2
• anti-inflammation

Glyceollins, produced to induce disease resistance responses against specific species, such as an incompatible pathogen Phytophthora sojae in soybeans, have the potential to exhibit anti-inflammatory activity in RAW 264.7 cells. To investigate the anti-inflammatory effects of elicited glyceollins via a signaling pathway, we studied the glyceollin signaling pathway using several assays including RNA and protein expression levels. We found that soybean glyceollins significantly reduced LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE(2)) production, as well as the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) via the suppression of NF-kappa B activation. Glyceollins also inhibited the phosphorylation of I kappa B alpha kinase (IKK), the degradation of I kappa B alpha, and the formation of NF-kappa B-DNA binding complex in a dose-dependent manner. Furthermore, they inhibited pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta and IL-18, but increased the generation of the anti-inflammatory cytokine IL-10. Collectively, the present data show that glyceollins elicit potential anti-inflammatory effects by suppressing the NF-kappa B signaling pathway in RAW 264.7 cells.

• 出版日期2012-4