Antibacterial, DNA interaction and cytotoxic activities of pendant-armed polyamine macrocyclic dinuclear nickel(II) and copper(II) complexes

作者：Arthi P; Haleel A; Srinivasan P; Prabhu D; Arulvasu C; Rahiman A Kalilur^*

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2014, 129: 400-414.

DOI：10.1016/j.saa.2014.03.058

• Pendant-armed macrocycles
• Dinuclear complexes
• Antibacterial activity
• DNA interaction
• Cytotoxicity
• Apoptosis

A series of dinuclear nickel(II) and copper(II) complexes (1-6) of hexaaza macrocycles of 2,6-diformyl-4-methylphenol with three different benzoyl pendant-arms, 2,2'-benzoyliminodi(ethylamine) trihydrochloride (L), 2,2'-4-nitrobenzoyliminodi(ethylamine) trihydrochloride (L') and 2,2'-3,5-dinitrobenzoyliminodi(ethylamine) trihydrochloride (L") have been synthesized and characterized by spectral methods. The electrochemical studies of these complexes depict two irreversible one electron reduction processes around E-pc(1) = -0.62 to -0.76 V and E-pc(2) = -1.21 to -1.31, and nickel(II) complexes (1-3) exhibit two irreversible one electron oxidation processes around E-pa(1) = 1.08 to 1.14 V and E-pa(2) = 1.71 to 1.74 V. The room temperature magnetic moment values (mu(eff), 1.52-1.54 BM) indicate the presence of an antiferromagnetic interaction in the binuclear copper(II) complexes (4-6) which is also observed from the broad ESR spectra with a g value of 2.14-2.15. The synthesized complexes (1-6) were screened for their antibacterial activity. The results of DNA interaction studies indicate that the dinuclear complexes can bind to calf thymus DNA by intercalative mode and display efficient cleavage of plasmid DNA. Further, the cytotoxic activity of complexes 2, 5 and 6 on human liver adenocarcinoma (HepG2) cell line has been examined. Nuclear-chromatin cleavage has also been observed with PI staining and comet assays.

• 出版日期2014-8-14