Deletion of the Scl+19 enhancer increases the blood stem cell compartment without affecting the formation of mature blood lineages

作者：Spen**erger Dominik; Kotsopoulou Ekaterini; Ferreira Rita; Broccardo Cyril; Scott Linda M; Fourouclas Nasios; Otter**ach Katrin; Green Anthony R; Goettgens Berthold

来源：Experimental Hematology, 2012, 40(7): 588-598.

DOI：10.1016/j.exphem.2012.02.006

The stem cell leukemia (Scl)/Tal1 gene is essential for normal blood and endothelial development, and is expressed in hematopoietic stem cells (HSCs), progenitors, erythroid, megakaryocytic, and mast cells. The Scl +19 enhancer is active in HSCs and progenitor cells, megakaryocytes, and mast cells, but not mature erythroid cells. Here we demonstrate that in vivo deletion of the Scl +19 enhancer (Scl(Delta 19/Delta 19)) results in viable mice with normal Scl expression in mature hematopoietic lineages. By contrast, Scl expression is reduced in the stem/progenitor compartment and flow cytometry analysis revealed that the HSC and megakaryocyte-erythroid progenitor populations are enlarged in Scl(Delta 19/Delta 19) mice. The increase in HSC numbers contributed to enhanced expansion in bone marrow transplantation assays, but did not affect multilineage repopulation or stress responses. These results affirm that the Set +19 enhancer plays a key role in the development of hematopoietic stem/progenitor cells, but is not necessary for mature hematopoietic lineages. Moreover, active histone marks across the Set locus were significantly reduced in Scl(Delta 19/Delta 19) fetal liver cells without major changes in steady-state messenger RNA levels, suggesting post-transcriptional compensation for loss of a regulatory element, a result that might be widely relevant given the frequent observation of mild phenotypes after deletion of regulatory elements.

• 出版日期2012-7
• 单位河北医科大学