TonB is essential for virulence in avian pathogenic Escherichia coli

作者：Holden Karen M; Browning Glenn F; Noormohammadi Amir H; Markham Philip F; Marenda Marc S^*

来源：Comparative Immunology, Microbiology and Infectious Diseases, 2012, 35(2): 129-138.

DOI：10.1016/j.cimid.2011.12.004

• ExPEC
• Respiratory infections
• Poultry
• TonB

Avian pathogenic Escherichia coli (APEC) strains have multiple iron-uptake systems that facilitate adaptation to iron-restricted environments and are believed to assist in colonisation of the host. These systems include several TonB-dependent transporters of ferri-siderophores encoded by the chromosome and the large virulence plasmid common to APECs. The tonB gene of the virulent APEC strain E956 was replaced with a selectable antibiotic resistance marker using Lambda Red recombinase mutagenesis. The phenotype of the tonB E956 mutant was compared to the parent strain under various culture conditions and in chickens experimentally infected via the respiratory route. The mutant was resistant to streptonigrin, impaired in its ability to adapt to growth in iron-depleted medium and had greater tolerance of oxidative stress than the parental strain. The mutant was avirulent in chickens, did not affect the growth of chicks and colonisation was mostly limited to the trachea. This study has demonstrated that TonB is essential for virulence in APEC.

• 出版日期2012-3