Murraya paniculata and Related Species as Potential Hosts and Inoculum Reservoirs of 'Candidatus Liberibacter asiaticus', Causal Agent of Huanglongbing

作者：Damsteegt V D^*; Postnikova E N; Stone A L; Kuhlmann M; Wilson C; Sechler A; Schaad N W; Brlansky R H; Schneider W L

来源：Plant Disease, 2010, 94(5): 528-533.

DOI：10.1094/PDIS-94-5-0528

Huanglongbing (HLB), considered to be the most serious insect-vectored bacterial disease of citrus is transmitted in nature by the Asian citrus psyllid Dtaphorina citri and the At man citrus Trioza erytreae D citri was discovered in southern Florida in 1998 and the HLB disease in 2005 Both have become established throughout citrus-producing areas of Florida Murraya species are widely grown in southern Florida as ornamental hedges and are readily colonized by D citri vectors Colonies of D citri. isolates of 'Candidatus Liberibacter asiaticus' from Taiwan and Florida. and the Murraya species were established in the BSL-3 biosecurity facility at Fort Detrick In controlled inoculation experiments. D citri transmitted 'Ca L asiaticus' into M paniculata (34/36 plants) and M exotica (22/23 plants), but not into Bergera (Murraya) koenign Disease symptoms rarely developed in Murraya plants. however positive infections were determined by conventional and real-tine polymerase chain reaction (PC R) Back-inoculations of 'Ca L asiaticus' front M paniculata to Madam Vinous sweet orange resulted in disease development in 25% of the inoculated plants Considerable variability was observed in infection rates, titer, and persistence of 'Ca L asiaticus' in infected Murraya