摘要
Huanglongbing (HLB), considered to be the most serious insect-vectored bacterial disease of citrus is transmitted in nature by the Asian citrus psyllid Dtaphorina citri and the At man citrus Trioza erytreae D citri was discovered in southern Florida in 1998 and the HLB disease in 2005 Both have become established throughout citrus-producing areas of Florida Murraya species are widely grown in southern Florida as ornamental hedges and are readily colonized by D citri vectors Colonies of D citri. isolates of 'Candidatus Liberibacter asiaticus' from Taiwan and Florida. and the Murraya species were established in the BSL-3 biosecurity facility at Fort Detrick In controlled inoculation experiments. D citri transmitted 'Ca L asiaticus' into M paniculata (34/36 plants) and M exotica (22/23 plants), but not into Bergera (Murraya) koenign Disease symptoms rarely developed in Murraya plants. however positive infections were determined by conventional and real-tine polymerase chain reaction (PC R) Back-inoculations of 'Ca L asiaticus' front M paniculata to Madam Vinous sweet orange resulted in disease development in 25% of the inoculated plants Considerable variability was observed in infection rates, titer, and persistence of 'Ca L asiaticus' in infected Murraya