科研之友
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摘要
To investigate the LHR protein expressions in the ovine uteri and LH secretion, and to determine whether the alarelin active immunity affects the uterine development in ewes. Twenty-eight ewes (five to six months) were assigned to four experimental groups. Animals in EG-1, EG-2 and EG-3 were subcutaneously injected with 200, 300 and 400 mu g alarelin antigens (day 0 and 14), respectively. Animals in the CG were injected with 2.0 mL solvent (day 0 and 14). Uterine horns were dissected aseptically on day 70. The serum LH concentrations were measured using ELISA. Tissue slices were observed and photographed under optical and electron microscopes. The images were measured using Images software. The results showed that the expressions of LHR protein in EGs increased. The level of LHR protein in EG-3 was higher than that in CG (P < 0.05). Serum LH concentrations in EGs were lower than that in CG from day 21 to day 45 (P < 0.05). Uterine weights in EG-1, EG-2 and EG-3 reduced by 4.66%, 10.20% and 16.63% (P<0.05), respectively. Uterine wall thickness (UWT) in EG-1, EG-2 and EG-3 reduced by 1.41%, 5.84% and 8.75% (P < 0.05), respectively. The endometrial epithelium thickness (EET) in EG-1, EG-2 and EG-3 decreased too (P < 0.05). The uterine wall shrank obviously and the glandular cavity reduced. The microvilli shortened. The mitochondria and mitochondrial crista decreased. Summarily, alarelin immunity could promote LHR protein expression in the uteri, and decreased serum LH concentrations, including decreased EET, UWT and uterine weights, could affect the uterine microstructure and ultrastructure, resulting in the inhibition of uterine development dose-dependently.
