Detection of erythrovirus B19 in thyroidectomy specimens from graves%26apos; disease patients: A case-control study

作者：Page Cyril; Hoffmann Thomas Walter; Benzerdjeb Nassim; Duverlie Gilles; Sevestre Henri; Desailloud Rachel^*

来源：Journal of Medical Virology, 2013, 85(8): 1414-1419.

DOI：10.1002/jmv.23595

• parvovirus B19
• erythrovirus B19 (EVB19)
• Graves%26apos
• disease
• autoimmune thyroiditis
• immunochemistry

Environmental factors, such as viruses, are thought to contribute to the development of thyroid autoimmunity. Erythrovirus B19 (EVB19) is suspected to be involved in Hashimoto%26apos;s thyroiditis, but no direct evidence is available concerning the role of EVB19 infection in Graves%26apos; disease. The objective of this study was to investigate whether the presence of EVB19 is more frequent in thyroidectomy specimens of patients undergoing thyroidectomy for Graves%26apos; disease (cases) than for multinodular thyroid (controls). Serum and thyroidectomy specimens were prospectively collected from 64 patients referred for total thyroidectomy over a 5-year period (2007-2011) and were investigated retrospectively and blindly for circulating EVB19 DNA by q-PCR (Qiagen), and for EVB19 thyrocyte infection by immunochemistry (VP2-Antibody, Dako). EVB19 serology was also determined. General clinical and laboratory data were collected. Twenty patients were referred for Graves%26apos; disease and 44 patients were referred for non-autoimmune multinodular thyroid. Patients with thyroid cancer were excluded. Ten percent of Graves%26apos; disease patients and 27.7% of control patients had positive staining of thyrocytes for EVB19 antibodies (ns). EVB19-positive and EVB19-negative cases did not differ. EVB19-positive controls were older than EVB19-negative controls (mean age: 57.5 [35-74] vs. 45 [28-80] years, P=0.03) No case of acute EVB19 infection was identified. EVB19-positive serology was more frequent in controls than in Graves%26apos; disease patients (88% vs. 45%, P%26lt;0.0001). EVB19 was detected in thyrocytes, but not more frequently in Graves%26apos; disease patients than in controls. Further studies are needed to determine the role of EVB19 infection in thyroid diseases.

• 出版日期2013-8