Use of Degradation Tags To Control Protein Levels in the Cyanobacterium Synechocystis sp Strain PCC 6803

作者:Landry Brian P; Stoeckel Jana; Pakrasi Himadri B*
来源:Applied and Environmental Microbiology, 2013, 79(8): 2833-2835.
DOI:10.1128/AEM.03741-12

摘要

We generated a collection of ssrA-based C-terminal protein degradation tags with different degradation strengths. The steady-state fluorescence levels of different enhanced yellow fluorescent protein (eYFP) tag variants in a Synechocystis sp. indicated a tunable range from 1% to 50% of untagged eYFP.

  • 出版日期2013-4