Systematic fractionation and immunoenhancement of water-soluble polysaccharides isolated from fruit of Morus alba L.

作者：Wang, DongXue; Li, Hui; Li, Bo; Ma, Rui; Zhang, Ning; Zhang, Xiaoyu; Jiao, Lili^*; Wu, Wei^*

来源：International Journal of Biological Macromolecules, 2018, 116: 1056-1063.

DOI：10.1016/j.ijbiomac.2018.05.106

• Fruit of Morus alba L.
• Polysaccharides
• Macrophages
• Immunostimulation

In this study, we obtained and fractionated systematically the polysaccharides of Fructus mori fruit by the sequential use of water extraction, ethanol precipitation and ion-exchange chromatography. The sugar contents of FPA-1, FPA-2, FPA-4 and FPA-5 were estimated to be 75.70%, 67.15%, 87.78% and 91.63%, respectively. Monosaccharide composition analysis revealed that all the polysaccharides primarily contained Man, GlcA, GalA, Glc, Gal, Xyl, Ara and Fuc. Moreover, a small amount of Rha was detected in FPA-1, FPA-4 and FPA-5. The molecular weight distributions of FPA-1, FPA-2, FPA-4 and FPA-5 were 10.6-49.1 kDa, 22.9-122.7 kDa, 57.1-105.4 kDa and 14.8-19.6 kDa, respectively. According to the in vitro experiment, FPA-1 exhibited the highest activity on enhancing phagocytosis, promoting macrophage to secrete nitric oxide, tumour necrosis factor-alpha and reactive oxygen species.

• 出版日期2018-9
• 单位长春中医药大学