ERG11 mutations are associated with high- level azole resistance in clinical Candida tropicalis isolates, a Singapore study

作者：Chew Ka Lip; Cheng Janet W S; Jureen Roland; Lin Raymond T P; Teo Jeanette W P^*

来源：Mycoscience, 2017, 58(2): 111-115.

DOI：10.1016/j.myc.2016.11.001

• Antifungal
• Drug resistance
• Molecular diagnostics
• Yeasts

Candida tropicalis is a commonly isolated non-albicans Candida in Asia. In this clinical microbiology laboratory study, a total of 1579 C. tropicalis were isolated during 2009-2014 and of these 348 isolates were tested for azole susceptibility. We show that the current rates of fluconazole resistance in C. tropicalis increased from 2.9% in 2009-2011 to 9% in 2012-2014 (P = 0.03). High fluconazole minimum inhibitory concentrations (MICs) were strongly associated with the presence of Y132F and S154F mutations in Erg11p. No amino acid changes were observed in Erg3p or Erg11p in sensitive isolates. This suggests that Y132F and S154F mutations were responsible for azole resistance. Multilocus sequence typing (MLST) based phylogenetic analysis performed for azole-resistant isolates suggested possible clonal clustering of antifungal-resistance.

• 出版日期2017-3