An Economical Method for Isolation of Dioscin from Dioscorea nipponica Makino by HSCCC Coupled with ELSD, and a Computer-Aided UNIFAC Mathematical Model

作者：Yin, Lian-Hong; Xu, Li-Na; Wang, Xiao-Na; Lu, Bi-Nan; Liu, Yue-Tao; Peng, Jin-Yong^*

来源：Chromatographia, 2010, 71(1-2): 15-23.

DOI：10.1365/s10337-009-1407-2

• Counter-current chromatography
• UNIFAC mathematical model
• Dioscin
• Dioscorea nipponica Makino

An economical method for isolation of dioscin from Dioscorea nipponica Makino by high-speed counter-current chromatography (HSCCC) was successfully developed by using a UNIFAC mathematical model coupled with computer-aided counter-current chromatography solvent-selection software (CCC-SSS) for separate preparation of the components of the solvent system (i.e., the stationary and mobile phases). The solvent system n-hexane-ethyl acetate-ethanol-water 2:5:2:5 (v/v) was selected to demonstrate the feasibility of the approach. A comparative study was also carried out on different methods for preparation of the solvent system, namely conventional preparation of the mobile and stationary phases together in the same vessel and the method developed for separate preparation of the phases. The results indicated that purity and recovery of dioscin were no different when solvent systems prepared by the different methods were used for HSCCC separation. Much less n-hexane, ethyl acetate, and ethanol was used when the mobile and stationary phases were prepared separately, however. This was not only environmentally sensible, but also enabled conservation of resources. Use of the UNIFAC mathematical model combined with the CCC-SSS technique for separate preparation of the components of the solvent system in HSCCC is reported and explained. It is a simple and economical means of isolating pure dioscin from Dioscorea nipponica Makino.

• 出版日期2010-1
• 单位大连医科大学