科研之友
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摘要
It was previously thought that arteriogenesis and venogenesis are induced not only by proliferation of vessel-resident smooth muscle cells (SMCs) and endothelial cells (ECs) but also by migration of their precursors. However, it is not well understood through what route(s) the precursors migrate into the existing vessels. %26lt;br%26gt;We examined through what route or routes circulating mononuclear cells expressing beta-actin (beta-MNCs), which we identified in canine coronary vessels, migrate into coronary vessel walls and cause arteriogenesis and venogenesis at 1, 2, 4 and 8 weeks after induction of myocardial infarction. %26lt;br%26gt;The following changes were observed: (1) The beta-MNCs migrated via coronary microvessels to the interstitial space at one week; (2) beta-MNCs traversed the adventitia into the media and settled in parallel with pre-existing smooth muscle cells (SMCs) in arterioles and arteries and lost beta-actin and acquired alpha-smooth muscle actin (alpha-SMA) to become mature SMCs at 2-4 weeks; (3) at the same time, other beta-MNCs migrated across the adventitia and media into the intima and settled in parallel with pre-existing endothelial cells (ECs) and lost beta-actin, while acquiring CD31, to become mature ECs, resulting in arteriogenesis; (4) Similarly, beta-MNCs migrated into venular and venous walls and became SMCs or ECs, resulting in venogenesis. %26lt;br%26gt;beta-MNCs in the interstitial space expressed CD34 but not other major vascular cell markers. %26lt;br%26gt;beta-MNCs, possibly a vascular progenitor, migrate not from the lumen but across the adventitia into the media or intima of coronary vessels and transit to SMCs or ECs, and participate in arteriogenesis and venogenesis in ischemic myocardium. (Int Heart J 2012; 53: 54-63)
- 出版日期2012-1
