科研之友
微信
新浪微博
Facebook
分享链接
摘要
A novel liquid-phase microextraction method coupled with high-performance thin-layer chromatography (HPTLC) for preconcentration, separation, and determination of six phenolic compounds in water samples has been developed. Target analytes were extracted using microliter volume of 1-undecanol, which floated on the surface of the aqueous sample and was separated on an RP-18 F(254) HPTLC plate using a mixture of methanol-water 2:1 (%, v/v) as the mobile phase. The plates were developed vertically up to a distance of 80 mm. The calibration curves were linear in the range of 0.025-4.0 and 0.1-20.0 mg per band, with good coefficient of determinations of 0.9978-0.9991 and 0.9985-0.9995, for phenol (PN), 2-chlorophenol (2-CP), 2,4-dichlorophenol (2,4-DCP), 2,4,6-trichlorophenol (2,4,6-TCP), 2,3,4,6-tetrachlorophenol (2,3,4,6-TeCP), and pentachlorophenol (PCP). The limit of detection (LOD) and limit of quantification (LOQ) values of the six investigated compounds were between 8-35 and 25-98 ng per band, respectively. The relative standard deviations for repeatability were between 2.61% and 6.35%. The accuracy of the method was checked by the determination of recovery at three different levels, and varied from 89.21% to 101.74% with RSD% of 2.07-6.73% (n = 3).
- 出版日期2011-6
