Molecular Identification of a New Wheat-Thinopyrum intermedium ssp trichophorum Addition Line for Resistance to Stripe Rust

作者：Song, X. J.; Li, G. R.; Zhan, H. X.; Liu, C.; Yang, Z. J.^*

来源：Cereal Research Communications, 2013, 41(2): 211-220.

DOI：10.1556/CRC.2013.0009

• in situ hybridization
• stripe rust resistance
• Thinopyrum intermedium ssp trichophorum
• wheat

Stripe rust, caused by Puccinia striiformis f. sp. tritici, was one of the most disaster foliar diseases for wheat-growing areas of the world. Thinopyrum intermedium has provided novel resistance genes to multi-fungal disease, and new wheat-Th. intermedium derivatives for stripe rust resistance still need to develop for wheat breeding. Wheat line X484-3 was selected from a cross between wheat line MY11 and wheat-Th. intermedium ssp. trichophorum partial amphiploid TE-1508, and was characterized by genomic in situ hybridization (GISH) and functional molecular markers. Chromosome counting revealed that the X484-3 was 2n = 44 and GISH analysis using Pseudoroegneria spicata genomic DNAas a probe demonstrated that X484-3 contained a pair of St-chromosomes from Th. intermedium donor parents. The functional molecular markers confirmed that introduced St-chromosomes belonging to linkage group 7, indicating that line X484-3 was a 7St addition line. The resistance observation displayed that the introduced Th. intermedium ssp. trichophorum derived chromosomes 7St were responsible for the stripe rust resistances at adult plant. The identified wheat-Th. intermedium chromosome 7St addition line X484-3 can be used as a donor in wheat breeding for stripe rust resistance.

• 出版日期2013-6
• 单位电子科技大学