科研之友
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摘要
Due to the different species of Epimedium separated by seasonal isolation and geography, developing a method for mid-term storage of E. pubescens is necessary to facilitate germplasm conservation, interspecific hybridization and identification of hybrid species. In order to optimize the process of hand-pollination, in this work we have studied the conservation potential of E. pubescens pollen stored for approximately 2 months at -20 degrees C, -70 degrees C and -196 degrees C. The optimal desiccation time of E. pubescens pollen was found to be 210 min. In vitro pollen germination of fresh pollen was 56.0%, and it was progressively reduced after conservation at -20 degrees C, -70 degrees C and -196 degrees C reaching a minimum of 4.2%, 6.0% and 4.4%, respectively after 2 months of storage. The germination capacity of pollen stored at -20 degrees C and -196 degrees C was similar to that observed at -70 degrees C, therefore for reasons of convenience and economy, pollen can be efficiently stored at -20 degrees C. There were no fruit set differences between the field pollinations performed with stored pollen and pollinations performed with fresh pollen. These results indicate that E. pubescens pollen can effectively stored at subzero temperatures for several weeks to months.
- 出版日期2012-4
- 单位西华师范大学
