Membrane-less microfiltration using inertial microfluidics

作者：Warkiani Majid Ebrahimi^*; Tay Andy Kah Ping; Guan Guofeng; Han Jongyoon

来源：Scientific Reports, 2015, 5(1): 11018.

DOI：10.1038/srep11018

Microfiltration is a ubiquitous and often crucial part of many industrial processes, including biopharmaceutical manufacturing. Yet, all existing filtration systems suffer from the issue of membrane clogging, which fundamentally limits the efficiency and reliability of the filtration process. Herein, we report the development of a membrane-less microfiltration system by massively parallelizing inertial microfluidics to achieve a macroscopic volume processing rates (similar to 500 mL/min). We demonstrated the systems engineered for CHO (10-20 mu m) and yeast (3-5 mu m) cells filtration, which are two main cell types used for large-scale bioreactors. Our proposed system can replace existing filtration membrane and provide passive (no external force fields), continuous filtration, thus eliminating the need for membrane replacement. This platform has the desirable combinations of high throughput, low-cost, and scalability, making it compatible for a myriad of microfiltration applications and industrial purposes.

• 出版日期2015-7-8