A fully electronic sensor for the measurement of cDNA hybridization kinetics

作者：Bandiera L^*; Cellere G; Cagnin S; De Toni A; Zanoni E; Lanfranchi G; Lorenzelli L

来源：Biosensors and Bioelectronics, 2007, 22(9-10): 2108-2114.

DOI：10.1016/j.bios.2006.09.025

• DNA sensors
• silicon nitride
• hybridization kinetic
• dsDNA
• cDNA

Ion sensitive field effect transistors (ISFET) are candidates for a new generation of fully electrical DNA sensors. To this purpose, we have modified ISFET sensors by adsorbing on their Si3N4 surface poly-L-lysine and single (as well as double) stranded DNA. Once coupled to an accurate model of the oppositely charged layers adsorbed on the surface, the proposed sensor allows quantitatively evaluating the adsorbed molecules densities, as well as estimating DNA hybridization kinetics.

• 出版日期2007-4-15