An efficient Agrobacterium tumefaciens mediated transformation and regeneration system for cotyledons of spinach (Spinacia oleracea L)

作者：Zhang HX; Zeevaart JAD^*

来源：Plant Cell Reports, 1999, 18(7-8): 640-645.

• spinach (Spinacia oleracea L.)
• regeneration
• green-fluorescent protein
• transformation
• cotyledon

An efficient transformation and regeneration system was established for the production of transgenic spinach (Spinacia oleracea L.) plants. Cotyledon explants were infected with Agrobacterium tumefaciens strain LBA4404 carrying the selectable marker gene, neomycin phospotransferase II (nptII), and the reporter gene smgfp, encoding soluble-modified green-fluorescent protein, driven by the cauliflower mosaic virus 35S promoter The infected explants were cultured on Murashige and Skoog medium, containing 1 mg/l benzyladenine and 0.4 mg/l naphthaleneacetic acid. Shoots were regenerated on selection medium containing 50 mg/l kanamycin. Regenerated kanamycin-resistant shoots were rooted on medium containing 1 mg/l indolebutyric acid and subsequently grown in soil in the greenhouse. Southern blot analysis indicated chat the smgfp gene bad been integrated into the spinach genome. Northern and Western blots showed that the smgfp gene was expressed in progeny plants.

• 出版日期1999-3