A transportable, inexpensive electroporator for in utero electroporation

作者:Bullmann Torsten*; Arendt Thomas; Frey Urs; Hanashima Carina
来源:Development Growth and Differentiation, 2015, 57(5): 369-377.
DOI:10.1111/dgd.12216

摘要

Electroporation is a useful technique to study gene function during development but its broad application is hampered due to the expensive equipment needed. We describe the construction of a transportable, simple and inexpensive electroporator delivering square pulses with varying length and amplitude. The device was successfully used for in utero electroporation in mouse with a performance comparable to that of commercial products.

  • 出版日期2015-6
  • 单位RIKEN