A PCR-RFLP method for typing human papillomavirus type 16 variants

作者：Lugo Trampe Angel; Trujillo Murillo Karina del C; Rodriguez Sanchez Iram P; Barboza Cerda Maria del C; Lugo Trampe Jose de J; Hernandez Ramirez Laura C; Canseco Avila Luis M; Espinoza Ruiz Marisol; Dominguez Arrevillaga Sergio; Delgado Enciso Ivan

来源：Journal of Virological Methods, 2013, 187(2): 338-344.

DOI：10.1016/j.jviromet.2012.10.013

• HPV 16
• Variants
• PCR-RFLP
• LCR
• Capillary electrophoresis

Infection with some types of human papillomavirus (HPV) is required for cervical cancer development, being HPV type 16 (HPV 16) the most common type in premalignant and malignant cervical lesions. DNA sequencing has revealed the existence of intratypic variants of HPV 16 whose genotyping is clinically useful for distinguishing between persistent and recurrent infections. From the epidemiological perspective, the frequency of diverse HPV 16 variants in several populations could correlate with the presence of precursor high-risk lesions in different anatomical locations. Currently, the %26quot;gold standard%26quot; method for identifying HPV 16 variants involves the sequencing of genomic regions to identify characteristic polymorphic sites. Although some other methods have been described, they require specialized or high-cost equipment. In this study, a robust and low cost procedure is described for HPV 16 variant typing, based on the long control region of the virus.

• 出版日期2013-2