Differential effects of paclobutrazol on water stress alleviation through electrolyte leakage, phytohormones, reduced glutathione and lipid peroxidation in some wheat genotypes (Triticum aestivum L.) grown in-vitro

作者：Aly Amina A^*; Latif Hanan H

来源：Romanian Biotechnological Letters, 2011, 16(6): 6710-6721.

• Mannitol
• paclobutrazol
• photosynthetic pigments
• phytohormones
• water stress
• wheat

This study was carried out to study the possibility of using paclobutrazol agent in order to decrease the adverse effect of water stress which was induced by mannitol. The main parameters of interest were; relative water content, electrolyte leakage, phytohormones, proline, reduced glutathione (GSH), malondialdehyde (MDA), total carbohydrates and pigments contents in seedlings of three wheat (Triticum aestivum L.) cultivars. Seeds of wheat cvs. (Sakha 8, Sakha 69 and Giza 168) were cultured on Murashige and Skoog (MS) medium supplemented with mannitol (Man) at 0.2M, paclobutrazol (PBZ), at 5 mg l(-1) concentration and combination between Man+PBZ for three weeks. Relative water content (RWC) declined as the Man treatment applied only or in combination with PBZ in all cultivars. Electrolyte leakage was increased by adding PBZ and Man stress treatments in wheat seedlings when compared to control treatment. Both PBZ and Man stress treatments changed the endogenous levels of plant hormones in all wheat cultivars. The contents of gibberellic acid (GA(3)) and indole-3-acedic acid (IAA) were decreased but abscisic acid (ABA) content was increased under both PBZ and Man treatments in all used cultivars, compared to control ones. The most significant increase in free proline was detected under Man+PBZ treatment in cultivar Sakha 8. Application of PBZ significantly changed the content of both GSH and MDA in all cultivars. Total carbohydrates content were significantly (P<0.05) increased in treated seedlings when compared to control one. Water-stress caused significant reduction (P<0.05) in total chlorophyll (73.96 %) and in total carotenoids (Cx+c) (83.3 2%) in Sakha 8, whereas application of PBZl significantly (P<0.05) increased Chl. a, b and carotenoids in all cultivars. The results of the present study suggested tha, the application of PBZ was partially effective in overcoming adverse effects of water stress induced by mannitol in wheat seedlings grown in-vitro.

• 出版日期2011-12