Diversity of seed storage proteins in common wheat (Triticum aestivum L.)

作者：Sramkova Zuzana; Gregova Edita^*; Slikova Svetlana; Sturdik Ernest

来源：Plant Genetic Resources: Characterization and Utilization , 2011, 9(2): 256-259.

DOI：10.1017/S1479262111000554

• high-molecular weight-glutenin subunits
• quality
• sodium dodecyl sulphate-polyacrylamide gel electrophoresis
• Triticum aestivum L
• wheat

The objective of our study was to determine the composition of high-molecular weight-glutenin subunits (HMW-GS) in 120 cultivars of common wheat (Triticum aestivum L.). Fourteen alleles and 34 allelic compositions were detected using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The most frequent HMW-GS alleles at the Glu-A1, Glu-B1 and Glu-D1 loci were null (57.1%), 7 + 9 (43.3%) and 5 + 10 (61.9%), respectively. However, low-frequency HMW-GS alleles were also observed, such as 13 + 16, 20, 21, 7 and 18, encoded by the Glu-B1 locus, and 4 + 12, encoded by the Glu-D1 locus. The wheat-rye 1BL.1RS translocation was identified in 25 cultivars, using acid polyacrylamide gel electrophoresis. The Glu-score varied greatly, and some lines reached the maximum value of 10.

• 出版日期2011-7