Toll-Like Receptor 7 Ligands Inhibit Influenza A Infection in Chickens

作者：Stewart Cameron R^*; Bagnaud Baule Audrey; Karpala Adam J; Lowther Sue; Mohr Peter G; Wise Terry G; Lowenthal John W; Bean Andrew G

来源：Journal of Interferon and Cytokine Research, 2012, 32(1): 46-51.

DOI：10.1089/jir.2011.0036

Avian influenza virus is endemic in many regions around the world and remains a pandemic threat, a scenario tied closely to outbreaks of the virus in poultry. The innate immune system, in particular the nucleic acid-sensing toll-like receptors (TLRs) -3, -7, -8, and -9, play a major role in coordinating antiviral immune responses. In this study we have investigated the use of TLR ligands as antivirals against influenza A in chickens. The TLR7 ligand poly-C inhibited low-path influenza A growth in the chicken macrophage cell line HD-11 more effectively than poly(I: C), which acts via TLR3. The TLR7 ligand 7-allyl-8-oxoguanosine (loxoribine) inhibited influenza A replication in vitro and in ovo in a dose-dependent manner. Treatment of primary chicken splenocytes with loxoribine resulted in the induction of interferons-alpha, -beta, and -lambda, and interferon-stimulated genes PKR and Mx. These results demonstrate that nucleic acid-sensing TLR ligands show considerable potential as antivirals in chickens and could be incorporated into antiviral strategies.

• 出版日期2012-1
• 单位CSIRO