Development of a real-time PCR method for the identification of wheat genotypes carrying different eyespot resistance genes

作者：Meyer N^*; Lind V; Karlovsky P; Zahn M; Friedt W; Ordon F

来源：Plant Breeding, 2011, 130(1): 16-24.

DOI：10.1111/j.1439-0523.2010.01808.x

• wheat
• eyespot
• Oculimacula spp
• resistance
• real-time PCR

P>Eyespot, caused by Oculimacula acuformis and O. yallundae, is an important disease of wheat (Triticum aestivum). The two pathogens generate similar symptoms and cannot be distinguished visually. In order to distinguish O. acuformis and O. yallundae infections and to identify reliable quantitative differences in the level of resistance of wheat genotypes, two SYBR Green based real-time PCR assays were developed. The influence of time after inoculation until sample collection, the effect of species (O. acuformis, O. yallundae), and the relevance of wheat cultivars carrying Pch1, Pch2, or none of the resistance genes on the pathogen-DNA content were analysed. The most clear-cut difference of pathogen content between resistant and susceptible wheat genotypes was obtained 12 weeks after inoculation with O. yallundae. Growth chamber tests using artificial inoculation with O. yallundae and sampling 12 weeks after inoculation for real-time PCR is shown to be a reliable procedure to determine the resistance level of wheat against eyespot. Likewise, this is the basis for the development of efficient molecular markers.

• 出版日期2011-2