ASSOCIATION OF INTERLEUKIN-1 BETA (+3954) GENE POLYMORPHISM AND GINGIVAL CREVICULAR FLUID LEVELS IN PATIENTS WITH AGGRESSIVE AND CHRONIC PERIODONTITIS

作者：Yucel O Ozer^*; Berker E; Mesci L; Eratalay K; Tepe E; Tezcan I

来源：Genetic Counseling, 2013, 24(1): 21-35.

• IL-1 beta-Aggressive periodontitis
• Chronic periodontitis
• Gene polymorphism
• GCF

Association of interleukin-1 beta (+3954) gene polymorphism and gingival crevicular fluid levels in patients with aggressive and chronic periodontitis: %26lt;br%26gt;Background and objective: The immune mechanisms and genetic variations that regulate genetic expression, production and biological activity of IL-1 beta, are thought to play an important role in the pathogenesis of periodontal disease. The aims of the present study were to analyse interleukin (IL)-1 beta (+3954) genotype and allele frequency in both chronic and aggressive periodontitis patients, and also to investigate whether this polymorphism is associated with gingival crevicular fluid (GCF) IL-1 beta levels, periodontal disease severity and clinical parameters in subjects of Turkish origin. %26lt;br%26gt;Methods: A total of 147 individuals were enrolled in the study including 56 aggressive periodontitis (AP), 44 chronic periodontitis (CP) patients and 47 healthy controls (C). Single nucleotide polymorphism at IL-1 beta (+3954) is analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). GCF samples were analyzed for IL-lp, using enzyme linked immunosorbent assay (ELISA). %26lt;br%26gt;Results: The distribution of genotypes and allele frequencies for IL-1 beta (+3954) were similar among the groups, in spite of a trend toward a higher frequency of allele 2 in the patient groups. The genotype distribution and allele frequencies were also not different after stratification of subjects according to the clinical attachment level (CAL%26lt;4mm and CAL%26gt;4mm). No differences were found between the GCF IL-1 beta levels of the different genotypes. Allele 2 was associated with increased bleeding on probing (BOP) sites in chronic periodontitis patients. %26lt;br%26gt;Conclusion: The results of this study do not support that genetic polymorphism in the IL-1 beta (+3954) could be identified as a susceptibility or severity factor in aggressive periodontitis, in the present population. The association of allele 2 frequency and higher percentage of BOP sites in chronic periodontitis suggest that IL-1 beta (+3954) potentially play a significant but not major role in the clinical outcome.

• 出版日期2013