An Electrochemiluminescent Method to Detect A Dipeptide Related to Diabetes

作者：Fang Chen; Zheng Li-Juan; Zhai Su-Yan; Tu Yi-Feng^*

来源：Chinese Journal of Analytical Chemistry, 2017, 45(12): 1951-1955.

DOI：10.11895/j.issn.0253.3820.171284

• Electrochemiluminescence
• Luminol
• Diabetes
• Glucagon-like peptide 1
• Dipeptide
• Dipeptidy1 peptidase IV

In this work, a simple and fast approach for dipeptide detection was developed. AuNPs were decorated onto the surface of indium tin oxide (ITO) glass to serve as the working electrode to trigger the electrochemiluminescence (ECL) of luminol. The property of this electrode was characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), electrochemistry and spectroscopic method. Under the optimum conditions, the dipeptide His. Ala could be detected within a linear range from 2.44 x 10(-11) mol/L to 1.22 x10(-7) mol/L, with a detection limit of 2. 42. x 10(-12) mol/L (S/N = 3). In human body, the glucagon. like peptide 1(GLP-1) might lose activity during degradation under the enzymatic action of dipeptidyl peptidase IV (PDD-IV), meanwhile release same concentrated His. Ala dipeptide. Thus, the detection of His. Ala dipeptide was of great significance for investigation of diabetes not only for understanding the relation between GLP-1, PDD-IV and its inhibitor, but also the drug discovering because of its potential availability as the target to control the blood GLP-1 level of type II diabetics.

• 出版日期2017-12
• 单位苏州大学