Near-full-length REV3L appears to be a scarce maternal factor in Xenopus laevis eggs that changes qualitatively in early embryonic development

作者：Ogawara Daichi; Muroya Taketo; Yamauchi Kazumi; Iwamoto Taka aki; Yagi Yoshihiko; Yamashita Yoshihiro; Waga Shou; Akiyama Masahiro^*; Maki Hisaji

来源：DNA Repair, 2010, 9(1): 90-95.

DOI：10.1016/j.dnarep.2009.10.004

• DNA polymerase zeta
• Translesion DNA synthesis
• Vertebrate
• Egg extracts
• Embryonic development

REV3 is the catalytic subunit of DNA polymerase zeta (pol zeta), which is responsible for the damage-induced mutagenesis that arises during error-prone translesion synthesis in eukaryotes. The related REV3L genes in human and mouse encode proteins of approximately 350 kDa, twice as large as yeast REV3, but full-length REV3L has not been identified in any vertebrate cell. We report that Xenopus laevis REV3L encodes a 352-kDa protein that has high overall amino acid sequence similarity to its mammalian counterparts, and, for the first time in a vertebrate species, we have detected putative REV3L polypeptides of 300 and 340 kDa in X laevis oocytes. Only the 300-kDa form is stored in eggs, where its concentration of about 65 pM is much lower than those of other replication and repair proteins including the accessory pol subunit REV7. In fertilized eggs, the levels of this polypeptide did not change until neurula; the larger 340-kDa form first appeared at stages after gastrula, suggesting a pattern of regulation during development. These observations indicate the existence of REV3L as a scarce protein, of approximately the full predicted size, whose level may impose severe constraints on the assembly of pol in X. laevis.

• 出版日期2010-1-2