Day/night patterns of melatonin and oxidative stress markers in the rat Harderian gland

作者：Djeridane Yasmina; Bonnefont Rousselot Dominique; Touitou Yvan^*

来源：Biological Rhythm Research, 2013, 44(6): 922-926.

DOI：10.1080/09291016.2013.781327

• Harderian gland
• melatonin
• thiobarbituric acid-reactive substances
• glutathione peroxidase
• superoxide dismutase
• stress markers

The rodent Harderian gland (HG) is a highly porphyrinogenic organ that is vulnerable since free radicals elicited by excessive porphyrins lead to oxidative damage. We assessed the day/night HG oxidative stress status by assaying lipid peroxidation products thiobarbituric acid-reactive substances (TBARS) and antioxidant defence systems: glutathione peroxidase (GPx), Cu, Zn-superoxide dismutase (SOD), and melatonin in male Wistar rats acclimatized to light/dark cycles regimen of 12:12 for 3weeks, before being sacrificed in the middle of the light phase or in the middle of the dark phase. Whereas TBARS concentrations were significantly higher during the photophase compared with scotophase (p%26lt;0.05), no day/night differences were noted in the endogenous cytoprotective and antioxidant agents, such as melatonin, GPx and SOD, in these organs. We show here that increased lipid peroxidation mostly during the light phase, a probable consequence of photoreaction, is not counteracted by HG melatonin and antioxidant enzymes GPx and SOD contents.

• 出版日期2013-12-1