Activation of the Double-stranded RNA-dependent Protein Kinase PKR by Small Ubiquitin-like Modifier (SUMO)

作者：de la Cruz Herrera Carlos F; Campagna Michela; Garcia Maria A; Marcos Villar Laura; Lang Valerie; Baz Martinez Maite; Gutierrez Sylvia; Vidal Anxo; Rodriguez Manuel S; Esteban Mariano; Rivas Carmen^*

来源：JOURNAL OF BIOLOGICAL CHEMISTRY, 2014, 289(38): 26357-26367.

DOI：10.1074/jbc.M114.560961

The dsRNA-dependent kinase PKR is an interferon-inducible protein with ability to phosphorylate the alpha subunit of the eukaryotic initiation factor (eIF)-2 complex, resulting in a shutoff of general translation, induction of apoptosis, and inhibition of virus replication. Here we analyzed the modification of PKR by the small ubiquitin-like modifiers SUMO1 and SUMO2 and evaluated the consequences of PKR SUMOylation. Our results indicate that PKR is modified by both SUMO1 and SUMO2, in vitro and in vivo. We identified lysine residues Lys-60, Lys-150, and Lys-440 as SUMOylation sites in PKR. We show that SUMO is required for efficient PKR-dsRNA binding, PKR dimerization, and eIF2 alpha phosphorylation. Furthermore, we demonstrate that SUMO potentiates the inhibition of protein synthesis induced by PKR in response to dsRNA, whereas a PKR SUMOylation mutant is impaired in its ability to inhibit protein synthesis and shows reduced capability to control vesicular stomatitis virus replication and to induce apoptosis in response to vesicular stomatitis virus infection. In summary, our data demonstrate the important role of SUMO in processes mediated by the activation of PKR.

• 出版日期2014-9-19